human stfr1
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ATCC
human stfr1
Human Stfr1, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and morehttps://www.bioz.com/product/human+stfr1/Salmonella+enterica+subsp%2E+enterica+(ex+Kauffmann+and+Edwards)+Le+Minor+and+Popoff+serovar+Paratyphi+A/pmc08799657-291-2-36
Average 92 stars, based on 38 article reviews
human stfr1 - by
Bioz Stars,
2026-09
92/100 stars
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1) Product Images from "Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections"
Article Title: Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections
Journal: Nature Communications
doi: 10.1038/s41467-021-27949-3
Figure Legend Snippet: MACV GP1-Fc immobilized onto anti-human Fc biosensor tips and incubated with ( a ) sTfR1 or ( b ) buffer alone, ( c ) sTfR1 in complex with Fab ch128.1/IgG1 or ( d ) Fab ch128.1/IgG1 alone, or ( e ) sTfR1 in complex with Tf or ( f ) Tf alone. The arrows indicate the time points at which the indicated proteins were added and the dissociation step. The experiment was performed twice with representative data from one of the experiments shown. Seconds (s). Source data are included in the Source Data file.
Techniques Used: Incubation
Related Articles
Clone Assay:Article Title: Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections
Article Snippet: .. DNA encoding human sTfR1 (GenBank: NM_003234.3 residues 121–760) was cloned into the pHLsec expression vector downstream of the pHLsec secretion signal. sTfR1 was produced using linear polyethylenimine (PEI) to transfect HEK 293 S GnTI −/− cells (ATCC ® CRL-3022TM) grown in suspension culture and maintained in FreeStyle 293 Expression Medium (Cat. No. 12338018) supplemented with 2% Ultralow IgG FBS (Cat. No. 16250078) and penicillin/streptomycin (Thermo Fisher Scientific). .. The supernatant was harvested 72 h post-transfection and sTfR1 purified using human Tf affinity chromatography as previously described , . sTfR1 eluted as a single peak in buffer containing 25 mM Tris-HCl (pH 7.5) and 150 mM NaCl by SEC on a Superdex ® 200 Increase column (GE Healthcare Life Sciences, Marlborough, MA, USA).
Expressing:Article Title: Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections
Article Snippet: .. DNA encoding human sTfR1 (GenBank: NM_003234.3 residues 121–760) was cloned into the pHLsec expression vector downstream of the pHLsec secretion signal. sTfR1 was produced using linear polyethylenimine (PEI) to transfect HEK 293 S GnTI −/− cells (ATCC ® CRL-3022TM) grown in suspension culture and maintained in FreeStyle 293 Expression Medium (Cat. No. 12338018) supplemented with 2% Ultralow IgG FBS (Cat. No. 16250078) and penicillin/streptomycin (Thermo Fisher Scientific). .. The supernatant was harvested 72 h post-transfection and sTfR1 purified using human Tf affinity chromatography as previously described , . sTfR1 eluted as a single peak in buffer containing 25 mM Tris-HCl (pH 7.5) and 150 mM NaCl by SEC on a Superdex ® 200 Increase column (GE Healthcare Life Sciences, Marlborough, MA, USA).
Plasmid Preparation:Article Title: Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections
Article Snippet: .. DNA encoding human sTfR1 (GenBank: NM_003234.3 residues 121–760) was cloned into the pHLsec expression vector downstream of the pHLsec secretion signal. sTfR1 was produced using linear polyethylenimine (PEI) to transfect HEK 293 S GnTI −/− cells (ATCC ® CRL-3022TM) grown in suspension culture and maintained in FreeStyle 293 Expression Medium (Cat. No. 12338018) supplemented with 2% Ultralow IgG FBS (Cat. No. 16250078) and penicillin/streptomycin (Thermo Fisher Scientific). .. The supernatant was harvested 72 h post-transfection and sTfR1 purified using human Tf affinity chromatography as previously described , . sTfR1 eluted as a single peak in buffer containing 25 mM Tris-HCl (pH 7.5) and 150 mM NaCl by SEC on a Superdex ® 200 Increase column (GE Healthcare Life Sciences, Marlborough, MA, USA).
Produced:Article Title: Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections
Article Snippet: .. DNA encoding human sTfR1 (GenBank: NM_003234.3 residues 121–760) was cloned into the pHLsec expression vector downstream of the pHLsec secretion signal. sTfR1 was produced using linear polyethylenimine (PEI) to transfect HEK 293 S GnTI −/− cells (ATCC ® CRL-3022TM) grown in suspension culture and maintained in FreeStyle 293 Expression Medium (Cat. No. 12338018) supplemented with 2% Ultralow IgG FBS (Cat. No. 16250078) and penicillin/streptomycin (Thermo Fisher Scientific). .. The supernatant was harvested 72 h post-transfection and sTfR1 purified using human Tf affinity chromatography as previously described , . sTfR1 eluted as a single peak in buffer containing 25 mM Tris-HCl (pH 7.5) and 150 mM NaCl by SEC on a Superdex ® 200 Increase column (GE Healthcare Life Sciences, Marlborough, MA, USA).
Suspension:Article Title: Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections
Article Snippet: .. DNA encoding human sTfR1 (GenBank: NM_003234.3 residues 121–760) was cloned into the pHLsec expression vector downstream of the pHLsec secretion signal. sTfR1 was produced using linear polyethylenimine (PEI) to transfect HEK 293 S GnTI −/− cells (ATCC ® CRL-3022TM) grown in suspension culture and maintained in FreeStyle 293 Expression Medium (Cat. No. 12338018) supplemented with 2% Ultralow IgG FBS (Cat. No. 16250078) and penicillin/streptomycin (Thermo Fisher Scientific). .. The supernatant was harvested 72 h post-transfection and sTfR1 purified using human Tf affinity chromatography as previously described , . sTfR1 eluted as a single peak in buffer containing 25 mM Tris-HCl (pH 7.5) and 150 mM NaCl by SEC on a Superdex ® 200 Increase column (GE Healthcare Life Sciences, Marlborough, MA, USA).
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